![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4940011
Designed by: Kuang Minjie Group: iGEM23_iZJU-China (2023-10-11)
Transcription unit of P450BM-3 QM-his and GlcDH-II-his
This is a transcription unit in pCDFDuet-1, of gene P450BM-3 QM-his and GlcDH-II-his. Transcription will be initiated through T7 promoter, and terminated by T7 terminator. Additionally, lac operator can control gene expression only possible when induced by IPTG, which promises biological safety.
Usage and Biology
More specific information can be found in BBa_K4940001 and BBa_K4940002.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1441
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1441
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1441
Illegal XhoI site found at 1952 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1441
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1441
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1287
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Categories
Parameters
None |