Composite

Part:BBa_K4940011

Designed by: Kuang Minjie   Group: iGEM23_iZJU-China   (2023-10-11)


Transcription unit of P450BM-3 QM-his and GlcDH-II-his

This is a transcription unit in pCDFDuet-1, of gene P450BM-3 QM-his and GlcDH-II-his. Transcription will be initiated through T7 promoter, and terminated by T7 terminator. Additionally, lac operator can control gene expression only possible when induced by IPTG, which promises biological safety.


Usage and Biology

More specific information can be found in BBa_K4940001 and BBa_K4940002.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1441
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1441
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1441
    Illegal XhoI site found at 1952
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1441
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1441
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1287


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Categories
Parameters
None